Human Cytomegalovirus-Encoded miR-US25-1 Aggravates the Oxidised Low Density Lipoprotein-Induced Apoptosis of Endothelial Cells
Figure 3
miR-US25-1 downregulated BRCC 3 by targeting the 5′ UTR. (a) BRCC 3 mRNA was reduced in EAhy926 cells after transfection with miR-US25-1 mimics. (b) and (c) Western blot analysis indicated that the expression of BRCC 3 was inhibited in protein level in EAhy926 cells by miR-US25-1 mimics transfection. (d) Schematic diagram of the pGL3-BRCC 3/BRCC reporter construction. (e) Relative luciferase activity of pGL3-BRCC 3/BRCC reporter or pGL-con vector in EAhy926 cells transfected with miR-US25-1 mimics. All experiments were performed in triplicate independently. Statistical significance was showed as and , ns: no significance.
