Human Cytomegalovirus-Encoded miR-US25-1 Aggravates the Oxidised Low Density Lipoprotein-Induced Apoptosis of Endothelial Cells
Figure 6
miR-US25-1 aggravated the deregulation of ox-LDL on the expression of LOX and cIAP-1 and the release of Cyt c in EAhy926 cells. (a)–(c) Influence of miR-US25-1 mimics transfection (50 nM) on the expression of LOX, cIAP-1, and Cyt c in mRNA levels in EAhy926 cells after 50 μg/mL ox-LDL treatment. (d) miR-US25-1 mimics transfection (50 nM) deteriorated the upregulation of the caspase 3 activity in EAhy926 cells by treatment of 50 μg/mL ox-LDL. (e) and (f) Western blot assay of LOX expression, cIAP-1 expression, and Cyt c release in EAhy926 cells which were treated with 50 μg/mL ox-LDL and transfected with 50 nM miR-US25-1 mimics or 50 nM miRNA control (β-actin as control). All results were got from triplicate independent experiments. Statistical significance was showed as and , ns: no significance.
