Cytokine Effects on Cell Viability and Death of Prostate Carcinoma Cells
Figure 4
Flow cytometry analysis of LNCaP cells using PI staining. (a) Effects of IL-13 (20 ng/mL), IL-1β (5 ng/mL), IFN-γ (25 ng/mL), LY-294002 (20 μM), BAY 11-7082 (30 μM) SP600125 (30 μM), SΒ203580 (30 μM), and UO126 (30 μM) on LNCaP cell death (sub-G1 fraction). Cells were left untreated for 72 h (ctrl), or treated with the indicated cytokine or inhibitor alone for 72 h, or pretreated with the indicated inhibitor for 1 h and subsequently treated with the indicated cytokine for a total of 72 h. Statistically significant differences are depicted as follows: () cytokine versus ctrl or inhibitor versus ctrl or inhibitor and cytokine versus ctrl (), (+) inhibitor and cytokine versus cytokine (), (#) inhibitor and cytokine versus inhibitor (). (b) Representative histograms of cell distribution according to their DNA content, as determined by flow cytometry using PI staining (M1 represents G1 phase, M2 S phase, M3 G2/S phase, and M4 sub-G1 fraction).
