Flow Cytometry Assessment of In Vitro Generated CD138+ Human Plasma Cells
Figure 1
CD138+ cells staining in newly generated plasma cells. Switched memory B lymphocytes were cultured for 24 days as described in M&M. The cells were stained with one of four PE-conjugated anti-CD138 mAbs, namely, B-A38, B-B4, DL-101, and MI-15, or with a mix of B-A38 and DL-101 (MIX). The cells were fixed, kept in the dark, and analyzed 18 h to 20 h later. CD138 expression profile obtained with B-A38 for one sample out of six independent experiments is compared in (a) with B-B4 and MI-15 staining and in (b) with DL-101 and the mix of DL-101 and B-A38 (MIX). (c) The median fluorescence intensity (MFI) for each CD138 staining condition is presented as the mean ± S.E.M. for B-B4 and MI-15 staining () and for B-A38, DL-101, and the MIX staining () as observed for in vitro generated plasma cells. No significant differences were obtained for MFI values for any possible comparisons. (d) The CD138+ cells frequency in the viable cells (Pacific blue negative) is presented as the mean ± S.E.M for each staining condition ( or as in panel (c)). Multiple comparisons were performed using a Bonferroni correction. Differences were observed between DL-101 and BA-38, B-B4, and MI-15 (indicated by **; ) as well as the MIX and BA-38, B-B4, and MI-15 (indicated by *; ).
