Flow Cytometry Assessment of In Vitro Generated CD138+ Human Plasma Cells
Figure 3
B-A38 and DL-101 are both present on bone marrow plasma cells. Four bone marrow samples were used to test the capacity of DL-101 to detect CD138+ cells. (a) The analysis was done on viable cells, using Sytox Blue exclusion, and then on gated cells, which represented less than 0.5% of total cells. (b) Profiles are showed for B-A38 and DL-101 PE-conjugated mAbs for one sample gated on region. The mean frequency ± S.E.M for CD138+ cells was 78.7% ± 1.2% for B-A38 and 59.3% ± 2.1% for DL-101. (c) Frequencies of total are shown for all bone marrow samples according to B-A38 and DL-101; the mean ± S.E.M. was 0.20% ± 0.06% and 0.11% ± 0.05%, respectively. No significant differences were observed between the B-A38 and DL-101 staining in these four bone marrow samples (nonparametric Kruskal-Wallis test).
