Flow Cytometry Assessment of In Vitro Generated CD138⁺ Human Plasma Cells

Figure 4

Titration of DL-101 and B-A38 staining on RPMI-8226. RPMI-8226 cells were stained with PE-conjugated DL-101 from eBioscience (DL-101a) and BioLegend (DL-101b) as well as B-A38. The manufacturer recommended volume was used as the reference (1, as indicated by arrow) and DL-101b and B-A38 were used at 0.25-, 0.5-, 1-, 2-, and 5-fold and DL-101a was used at 0.20-, 0.4-, 1-, 2-, and 4-fold. SI was calculated as described in Table 1.