Target Capture and Massive Sequencing of Genes Transcribed in Mytilus galloprovincialis

Table 3

Enrichment fold and coverage of selected transcripts.

ID	Status	Enrichment fold (RT_PCR)	Sequenced reads (NGS)
MGC04518	On-target	32	22
MGC00300	On-target	60	45
MCG05878	On-target	2	7
Target 4	Off-target	−867	/

Enrichment real-time analysis was performed on 3 targets and on 1 not selected transcript (target 4).
Enrichment fold was measured in qRT-PCR by comparing the DNA library before and after enrichment and, subsequently, by reporting the number of reads that mapped uniquely on the targets.