Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes

Figure 1

Human astrocytes respond to albumin increasing cytosolic calcium concentration. (a) Effects of BPA onto [Ca²⁺]_𝑐 at different concentrations from 0.1 to 80 mg/mL. (b) Mean height of [Ca²⁺]_𝑐 in response to increasing BPA concentrations (solid dots, 𝑛=72 cells obtained from 8 different patients). Data are fitted (solid line) to the function𝑓(BPA)=−0.07+0.55(1−𝑒^−BPA/20.49); 𝑟=0.990. Solid arrowhead shows the EC₅₀ at 14.5 mg/mL. Empty dots represent [Ca²⁺]_𝑐 responses to HPA (𝑛=23 cells from 4 patients). Data are fitted (dashed line) to the function (HPA)=0.03+0.49(1−𝑒^−HPA/22.22); 𝑟=0.979. Empty arrowhead shows the EC₅₀ at 13.8 mg/mL. (c) A similar effect onto [Ca²⁺]_𝑐 was observed using either BPA (left) or human plasma albumin (HPA) obtained from the patient (right) at the same concentration (20 mg/mL). Lower empty bars show time of albumin application (in min). Concentrations are given in mg/mL.

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