Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes

Figure 5

DNA synthesis induced by plasma albumin. (a) Effect of different BPA concentrations onto DNA synthesis. (b) Comparison of normalized [Ca²⁺]_𝑐 (empty dots) and DNA synthesis (solid dots) induced by BPA. The [Ca²⁺]_𝑐 was fitted to an exponential function (𝑓(PBA)=103.9(1−𝑒^−BPA/21.74);𝑟=0.994) and DNA synthesis to an sigmoid function (𝑓(BPA)=0.08+100.5/(1+𝑒^{−(BPA−6.0)/1.83}); 𝑟=0.998) by the least-square method. Solid arrowhead indicates the EC₅₀ for [Ca²⁺]_𝑐 increases (14.5 mg/mL) and solid arrow marks the EC₅₀ for DNA synthesis (10.3 mg/mL). (c) DNA synthesis is partially blocked by LY-364947 (30 μM) and heparin (1 mg/mL). Combination of heparin + LY-364947 reduces in a higher degree DNA synthesis; however, it keeps some amount of DNA synthesis induced by plasma albumin. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 for Student’s 𝑡-test.

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