Apoptosis Induction by Polygonum minus Is Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line
Table 2
Antioxidant activities of fractions isolated from EtOAc crude extract of P. minus.
EtOAc fractions
TPC (mg GAE/g extract)
DPPH scavenging activity (EC50) (μg/mL)
FRAP value μmol Fe (II)/mg dry weight extract)
F1
ND
>1 mg/mL
ND
F2
14.2 ± 3.5a
568 ± 8.7a
147.53 ± 8.4a
F3
16.3 ± 4.2a
348.6 ± 5.7b
187.46 ± 10.7b
F4
21.7 ± 3.1b
200.2 ± 2.1c
224.41 ± 26.5c
F5
57.2 ± 6.8c
140.3 ± 3.2d
270.76 ± 17.2d
F6
96 ± 6.7d
90.2 ± 4.5e
356.84 ± 10.9e
F7
113.16 ± 6.2e
30.5 ± 3.2f
1169.1 ± 20.3f
Trolox
99 ± 2.4d
ND
ND
Chlorogenic acid
ND
19.95 ± 2.8g
ND
Ascorbic acid
ND
ND
3305.67 ± 51.5g
Values are mean ± S.D (). Values with different alphabets in the same column are significantly different (P < 0.05, ANOVA and Bonferroni test). GAE: gallic acid equivalents. ND: not determined.