Apoptosis Induction by Polygonum minus Is Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line

Table 4

IC₅₀ values of P. minus extracts on different cell lines after 72 h of incubation.

Crude extract	HepG2	WRL 68	HeLA	HCT 116	MCF-7	Chang
	IC50 (μg/mL)
Pet. ether	>250^a	56.23 ± 3.2^c	127.21 ± 3.2^b	145.32 ± 3.3^d	>250^a	>250^a
EtOAc	32.25 ± 3.72^b	122.38 ± 20.84^b	63.09 ± 6.7^c	199.52 ± 5.2^b	>250^a	285.72 ± 59.8^b
MeOH	>250^a	>250^a	205 ± 4.3^d	86.3 ± 3.5^c	>250^a	>250^a
Water	>250^a	>250^a	>250^a	>250^a	>250^a	>250^a

HepG2 cells (2 × 10⁶/well) were incubated with crude extracts of P. minus for 72 h at 37°C. Results are mean ± S.D (𝑛=3). Values with different alphabets in the same column are significantly different (P < 0.05, ANOVA and Bonferroni test).