HPLC-DAD Analysis, Antileishmanial, Antiproliferative, and Antibacterial Activities of Lacistema pubescens: An Amazonian Medicinal Plant

Figure 1

Chromatogram of methanolic extract of L. pubescens leaves. Peaks identified: 1, phytol (TR = 4.8 min), and 2, sitosterol (TR = 18.5 min). HPLC conditions: Zorbax SB-18 (250 mm × 4.6 mm i.d.; 5 μm); methanol-acetonitrile (70 : 30 v/v); 1.0 mL/min; injection volume, 20 μL; UV detection at 210 nm.