NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways
Figure 6
The effect on human skin fibroblast migration of blocking JNK, ERK, or PI3K/Akt pathways with specific inhibitors. ((A1), (B1), and (C1)) The levels of Akt, JNK, and ERK activity were all enhanced after treatment with 100 ng/mL of NGF for 5, 15, or 30 min. ((A2), (B2), and (C2)) The activity of Akt, JNK, or ERK following 5 min of NGF treatment was abolished by the corresponding specific inhibitor: LY294002 (10 μM, LY is the abbreviation for LY294002), SP600125 (10 μM, SP is the abbreviation for SP600125), or PD98059 (10 μM, PD is the abbreviation for PD98059). ((A3), (A4), (B3), (B4), (C3), and (C4)) The NGF-induced migration of human skin fibroblasts induced by NG was significantly impaired after incubation with LY294002 (10 μM), SP600125 (10 μM), or PD98059 (10 μM). , compared to the control group. ((D1) and (D2)) Active Rac1 was pulled down and then detected by Western blotting. , compared to untreated control group.
