Experience of Preimplantation Genetic Diagnosis with HLA Matching at the University Hospital Virgen del Rocío in Spain: Technical and Clinical Overview
Table 1
Results of the HLA or PGD/HLA cycles performed in HUVR.
Couple
Mode of transmission
Theoretical probability of suitable embryos
Cycle
Oocytes retrieved
Mature oocytes injected
Fertilized oocytes
Analyzed embryos
Diagnosed embryos
Genetically suitable embryos*
Transferred embryos
Pregnancy/Clinical pregnancy
1 (-THALASSEMIA)
Autosomal Recessive
3/16 (18.75%)
1
20
17
14
11
11
1
1
No
2
25
22
18
17
17
2
1
Yes/Yes
Overall
45
39 (86.6%)
32 (82%)
28 (87.5%)
28 (100%)
3 (10.7%)
2
2 (-THALASSEMIA)
Autosomal Recessive
3/16 (18.75%)
1
9
5
5
0
0
0
0
—
2
9
2
2
2
2
0
0
—
3
10
6
5
5
5
0
0
—
Overall
28
13 (46.4%)
12 (92.3%)
7 (58.33%)
7 (100%)
0 (0%)
0
—
3 (ADA)
Autosomal Recessive
3/16 (18.75%)
1
29
23
12
9
9
0
0
—
2
19
17
12
12
11
0
0
—
3
23
20
9
9
9
0
0
—
4
18
18
12
8
8
2
0
—
5
17
14
11
10
9
0
0
—
Overall
106
92 (86.8%)
56 (60.87%)
48 (85.7%)
46 (95.83%)
2 (4.4%)
0
4 (-THALASSEMIA)
Autosomal Recessive
3/16 (18.75%)
1
11
11
4
4
4
0
0
—
2
19
13
7
7
7
0
0
—
3
20
16
8
8
5
0
0
—
4
29
22
12
11
11
1
1
No
5
18
16
9
8
8
0
0
—
6
14
11
7
7
6
1
1
Yes/No
7
18
14
9
7
7
1
0
—
8
29
15
7
6
6
0
0
—
9
30
27
10
10
10
4
2
No
Overall
188
143 (76.0%)
73 (51.1%)
68 (93.2%)
64 (94.1%)
7 (10.9%)
4
5 (β-THALASSEMIA)
Autosomal Recessive
3/16 (18.75%)
1
8
4
3
3
3
0
0
—
2
16
14
6
6
6
0
0
—
Overall
24
18 (75%)
9 (50%)
9 (100%)
9 (100%)
0 (0%)
0
—
6 (BONE MARROW APLASIA)
Acquired
1/4 (25%)
1
29
28
14
14
13
3
2
No
2
34
27
19
19
18
1
1
Yes/Yes
Overall
63
45 (71.4%)
33 (73.3%)
33 (100%)
31 (93.9%)
4 (12.9%)
3
7 (DIAMOND-BLACKFAN ANEMIA)
De novo
1/4 (25%)
1
12
9
4
4
4
0
0
—
2
14
11
6
3
3
0
0
—
3
15
12
4
2
2
0
0
—
Overall
41
32 (78.0%)
14 (43.8%)
9 (62.3%)
9 (100%)
0 (0%)
0
Cycles in which molecular diagnosis and HLA-typing were performed on 2 cells biopsied from each embryo, are in italic. For the remaining cycles, just 1 blastomere was biopsied per embryo.
*“Genetically suitable embryos” were those non-affected embryos that were HLA-identical to the affected patient within the context of each family.
