An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the Leishmania Spp. and Trypanosoma cruzi Host-Parasite Relationship
Figure 2
Cover of Memórias do Instituto Oswaldo Cruz, vol. 86 (1) [14]. Likely the first DIC image obtained with a confocal microscope (W. Brad Amos, personal communication) showing HeLa cell infection by G strain metacyclic trypomastigotes. On the right, the corresponding image after immunofluorescence with monoclonal antibody anti-35/50 kDa mucin, suggesting release of the molecule in parts of internalized parasites [15].
