An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the <i>Leishmania</i> Spp. and <i>Trypanosoma cruzi</i> Host-Parasite Relationship

<table>Actin recruitment by EAs in the phagocytic cup (Supplementary Video 2 available online at http://dx.doi.org/10.1155/2014/565291). HeLa cells transfected with fluorescent actin marker were incubated with EAs (arrow) and observed by time-lapse confocal microscopy (Leica SP5 TS) for 30 minutes at one frame per 57 seconds. Total EA internalization occurred within approximately 4 minutes (*), but actin mobilization diffused approximately 9 minutes after total EA internalization (**) 13 minutes after recording initiation. Actin is shown in red (Life-actin, ibidi); EA nucleus and kinetoplast are shown in blue (Hoescht 33258). Scale bar, 5 <i>μ</i>m.</table>

BioMed Research International

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Figure 3

Figure 3: An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the <i>Leishmania</i> Spp. and <i>Trypanosoma cruzi</i> Host-Parasite Relationship 

Figure 3 | An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the Leishmania Spp. and Trypanosoma cruzi Host-Parasite Relationship 