An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the Leishmania Spp. and Trypanosoma cruzi Host-Parasite Relationship
Figure 7
Visualization of host cell cytoskeleton networks and intracellular amastigotes of T. cruzi (a, b) and L. amazonensis (c, d) with host cell cytoskeleton networks. Infected HeLa cells (a, b) and mouse bone marrow macrophages (c, d) were treated with a membrane extraction solution containing Triton X-100, taxol, and phalloidin (to stabilize microtubules and microfilaments) [16, 17]. Cytoskeletons of infected cells were visualized by field emission scanning electron microscopy after processing and gold coating. Scale bars: (a) 20 μm; (b) 3 μm; (c) 30 μm; (d) 5 μm.
