BioMed Research International

Research Article

Contrasted Reactivity to Oxygen Tensions in Frankia sp. Strain CcI3 throughout Nitrogen Fixation and Assimilation

Table 1

Primers used in this study.
Locus tagGeneGene identity Sequence
francci3_4488 nifHNitrogenase reductase iron-sulfur protein5′-CGACAACGACATGAAGACC-3′ 
5′-CTTGCCGATGATGCTCTC-3′
francci3_4487 nifDNitrogenase molybdenum-iron protein alpha chain 5′-AAGGACATCGTCAACATCAGCCAC-3′ 
5′-AACTGCATCGCGGCGAAGTTATTC-3′
francci3_4486 nifKNitrogenase molybdenum-iron protein beta chain 5′-TGACGACGACTCCGGAAACAAACA-3′ 
5′-TGTGGTAGACCTCGTCCTTGAACA-3′
francci3_4496 hup1Nickel-dependant hydrogenase, large subunit 5′-AACAAATCTGCGACGTCACGGTCA-3′ 
5′-ACTCTCGATCCATTCACCGCAGTA-3′
francci3_1076 hup2Uptake hydrogenase, large subunit 5′-TGGAAGGTCAACTGGCTGGAGAA-3′ 
5′-ATGTCTAGGCAGTACCGGAGGAAGAA-3′
francci3_1149 hboOTruncated hemoglobin 5′-GGGACGCCTGGCTGAAGA-3′ 
5′-CCAGAGCTGCCTGTCGAGATC-3′
francci3_2581 hboNTruncated hemoglobin5′-CACCCCTCTTTGCCAACCG-3′ 
5′-GGTGGTTTCCGTCGGGAC-3′
francci3_0823 sqhCSqualene hopene cyclase 5′-TGCAATGGCTGCTGGACAA-3′ 
5′-TGCCGTAGACGTGGTTGAT-3′
francci3_0819 hpnCSqualene synthase 5′-AACTTCCCGGTCTCGCCGTT-3′ 
5′-AACGCGTTGAAGTGGAAACGAACC-3′
francci3_2949 katACatalase 5′-ACATGCCGGTGTTCTTCATTCAGG-3′ 
5′-ACATCATCATGTGGCATCGACTCGG-3′
francci3_2817 sodASuperoxide dismutase 5′-GTGCCAATGACACCCTTGAGAAGA-3′ 
5′-AGTGGAGAATATGCCCGGAAAGGT-3′
francci3_3012 gltDGlutamate synthase, small subunit5′-TGCATGCGACGAACAACTTCCC-3′ 
5′-ATGATGCTGACCTCGATCTGCTTG-3′
francci3_3013 gltBGlutamate synthase, large subunit5′-CGTGCTGAAGGTGATGTCCAAGAT-3′ 
5′-AAATAGGCGTCGATCAGTTCCTGG-3′
francci3_3142 glnAGlutamine synthetase, type I 5′-ATGACCCGATCACCAAGGAACAGT-3′ 
5′-GGGTTGTAGTCATAACGGACATCG-3′
francci3_3143 glnAGlutamine synthetase, type II 5′-AACTTCTCCACCAGGCAGACGAT-3′ 
5′-AGAACTTGTTCCACGGAGCTGTCT-3′
francci3_4059 glnAGlutamine synthetase, catalytic region 5′-TACAACATCGACTACGCGCTTTCC-3′ 
5′-ATACCGGAACACGATCTCGAACTG-3′
francci3_1057 rpsA30S ribosomal protein S1 5′-CGAAGTCCGTTCCGAGTTC-3′ 
5′-CGCCGAAGTTGACGATGG-3′
Locus tag and gene designation were determined from the Integrated Microbial Genomes System (IMG) at the Joint Genome Institute (https://img.jgi.doe.gov/) [51].