Biological and Molecular Effects of Small Molecule Kinase Inhibitors on Low-Passage Human Colorectal Cancer Cell Lines
Figure 2
SMI-dependent death of HROC24 cells. HROC24 cells growing in 12-well plates were treated with SMI and combinations thereof for 48 h as indicated. Afterwards, they were subjected to (a) cytofluorometric quantification of dead (PI-positive) cells, (b) determination of cell death by trypan blue staining, and (c) detection of apoptotic (Sub-G1 peak) cells. The portion of dead/apoptotic cells is expressed as percent of the total cell count. Data from separate cultures were used to calculate mean values ± SEM. (a): versus control cells cultured without SMI and versus cells cultured with single SMI with Bonferroni-adjusted ; (b): versus control cultures with Bonferroni-adjusted ; (c): versus control cultures with Bonferroni-adjusted .
