Biological and Molecular Effects of Small Molecule Kinase Inhibitors on Low-Passage Human Colorectal Cancer Cell Lines
Figure 4
Quantitative analysis of the effects of vemurafenib, trametinib, perifosine, and regorafenib on signal transduction in CRC cell lines. The effects of (a) vemurafenib, (b) trametinib, (c) perifosine, and (d) regorafenib on fluorescence signal intensities of phosphoproteins (P-AKT, P-MEK1/2, and P-ERK1/2, resp.) and corresponding total proteins in HROC18 (black), HROC24 (grey), HROC43 (red), and HROC46 (orange) cells were quantified. Subsequently, the ratios P-MEK/MEK protein (left panels), P-ERK/ERK protein (middle panels), and P-AKT/AKT protein (right panels) were determined. A ratio of 1 corresponds to control cells cultured without SMI. Data of 5 independent experiments were used to calculate mean values ± SEM; versus control cultures with Bonferroni-adjusted .
