Recent Progress in Cryopreservation of Bovine Oocytes
Figure 3
Effect of ROCK inhibition during postwarm recovery culture on revivability of bovine mature oocytes [85]. (a) Proportion of vitrified-warmed bovine oocytes exhibiting the formation of no, single, or multiple sperm aster(s). The abnormal incidence of multiple aster formation was inhibited by the recovery culture with Y-27632. Immunostaining against α-tubulin (green) and nuclear staining with DAPI (blue) were performed at 10-hour post-insemination (hpi). (b) Accelerated timing of first cleavage in bovine oocytes vitrified-warmed and rescued with Y-27632. Asterisks indicate significant difference at .
