Review Article

Recent Progress in Cryopreservation of Bovine Oocytes

Table 1

A list on Day-2 cleavage and Day-8 blastocyst yield from bovine mature oocytes cryopreserved and fertilized in vitro.

YearsMethod/device
for cryopreservation
Oocyte cryosurvivalLiterature
Cleavage rateBlastocyst yield

1992 Freezing/French straw42%3%Otoi et al. [45]
1992 Vitrification/French straw22%9%Hamano et al. [46]
1996 Vitrification/EM-grid*140% 15%Martino et al. [47]
1998 Vitrification/OPS*250%13%Vajta et al. [48]
2000 Vitrification/Microdrop62%11%Dinnyes et al. [49]
2004 Vitrification/Cryotop70%7%Chian et al. [50]
2005 Vitrification/GL-tip*349%17%Tominaga et al. [51]
2010 Vitrification/Cryotop76% 12%Zhou et al. [52]

EM-grid: electron microscope grid; OPS: open-pulled straw; GL-tip: gel-loading tip.