Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample

Table 1

Variables studied using Plackett-Burman design.

Variable	Code	High value (+1)	Low value (−1)
NaCl (g/L)	Y1	80	40
Yeast extract (YE) (g/L)	Y2	10	1
MgSO4 (g/L)	Y3	9.6	0.9
MgCl2 (g/L)	Y4	7	0.7
Protease peptone (P.Pep.) (g/L)	Y5	5	0.5
KCl (g/L)	Y6	2	0.2
Glucose (g/L)	Y7	10	1
CaCl2 (g/L)	Y8	0.3	0.03
Temperature (°C)	Y9	25	15
pH	Y10	8.5	6.5