Research Article
Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample
Table 2
Plackett-Burman design for evaluating the significant variables for PUFA production by Kocuria sp. BRI 35.
| | Run | Y1 | Y2 | Y3 | Y4 | Y5 | Y6 | Y7 | Y8 | Y9 | Y10 | Dummy | PUFA production
(% of total fatty acids)
mean ± standard error |
| | 1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | 20.5 ± 0.56 | | 2 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | 1.0 ± 0.16 | | 3 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | 1.69 ± 0.08 | | 4 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | 0.95 ± 0.20 | | 5 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | 13.29 ± 0.62 | | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 ± 0.06 | | 7 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | 1.36 ± 0.41 | | 8 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | 22.37 ± 0.61 | | 9 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | 2.51 ± 0.59 | | 10 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | 2.57 ± 0.26 | | 11 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | 7.94 ± 0.68 | | 12 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | +1 | +1 | +1 | −1 | 1.83 ± 0.50 | | 13 | +1 | +1 | +1 | −1 | −1 | −1 | +1 | −1 | +1 | +1 | −1 | 3.25 ± 0.47 |
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