Statistical Approach for Production of PUFA from Kocuria sp. BRI 35 Isolated from Marine Water Sample

Table 5

CCD matrix of variables with response.

Run	MgSO4	Protease peptone	Glucose	Temperature	PUFA production
					(% of total fatty acids)
					[Mean ± standard error]
					Actual values	Predicted values
1	−1	+1	−1	−1	57.86 ± 0.92	58.45
2	−1	−1	−1	+1	10.11 ± 0.96	9.48
3	+1	+1	−1	−1	38.13 ± 1.19	33.80
4	0	0	0	+2	11.78 ± 0.79	4.6
5	0	0	0	0	17.26 ± 0.34	17.25
6	+1	+1	+1	−1	6.53 ± 0.46	13.86
7	+1	−1	+1	−1	18.57 ± 0.60	11.90
8	0	0	0	0	16.78 ± 0.85	17.25
9	+1	+1	+1	+1	2.32 ± 0.30	0.98
10	0	0	0	0	19.6 ± 0.52	17.25
11	0	0	+2	0	44.81 ± 0.86	42.76
12	−1	−1	−1	−1	9.97 ± 0.09	8.70
13	+1	+1	−1	+1	7.96 ± 0.05	18.13
14	−1	+1	+1	+1	11.42 ± 0.29	14.22
15	+2	0	0	0	29.81 ± 0.41	26.49
16	+1	−1	+1	+1	42.66 ± 0.86	48.69
17	−2	0	0	0	31.25 ± 0.19	30.54
18	0	−2	0	0	14.52 ± 0.20	19.01
19	+1	−1	−1	+1	56.26 ± 0.45	51.88
20	0	+2	0	0	29.57 ± 0.55	21.04
21	−1	−1	+1	+1	26.37 ± 0.68	28.09
22	0	0	0	0	16.29 ± 0.24	17.25
23	−1	+1	+1	−1	58.62 ± 0.35	60.39
24	0	0	0	0	18.35 ± 0.51	17.25
25	0	0	−2	0	45.99 ± 0.49	44.01
26	0	0	0	0	15.28 ± 0.42	17.25
27	+1	−1	−1	−1	14.07 ± 0.08	17.89
28	−1	+1	−1	+1	5.42 ± 0.52	9.48
29	−1	−1	+1	−1	28.15 ± 1.70	24.60
30	0	0	0	−2	13.65 ± 0.28	16.79