Downregulation of Transketolase Activity Is Related to Inhibition of Hippocampal Progenitor Cell Proliferation Induced by Thiamine Deficiency

Figure 1

Effect of thiamine deficiency on the proliferation of hippocampal progenitor cells (HPCs). Neurospheres of the thiamine deficiency (TD) group ((b1), 4 d of culture; (b2), 7 d of culture) were much smaller than those of the control group ((a1), 4 d of culture; (a2), 7 d of culture). On the seventh day of culture, the structure of the neurospheres of the TD group became porous, but their size increased and their structure became tight after replenishment of thiamine (b3). The optical density (OD) value of the TD group in the CCK-8 assay, indicating viability, was significantly lower than that of the control group by the fourth day (c). Scale = 100 μm.