Research Article

TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity

Table 1

Bacterial strains and plasmids.

Strain or plasmidDescriptionSource or reference

Strain
 ExPEC strain PPECC42Wild-type (WT), porcine origin, CmSLab stock
 PPECC42tolC Mutant with a 158-bp fragment deleted from the whole ORF of the tolC gene in PPECC42, CmSThis study
 Cm-tolC PPECC42tolC strain containing plasmid pHSG-tolC, CmRThis study
χ7213Thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu[λpir] KmR[22]
χ7213-tolC χ7213 containing suicide vector pRE112ΔtolC This study
 DH5αF, φ80dlacZΔM15, (lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(, ), phoA, supE44, λ , thi-1, gyrA96, relA1 Takara Bio
Plasmid
pRE112 oriT oriV Δasd CmR SacB, suicide vector[22]
pRE tolC pRE112-inserted disrupted tolC gene in Kpn I and Sac I sitesThis study
pHSG396 ori lacZ CmRTakara Bio
pHSG-tolC CmRThis study