TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity

Table 2

Primers for PCR/RT-PCR.

Primer^a	Sequence (5′-3′)	Application	Source or reference
tolC-P1	GCCGGTACCATGAAGAAATTGCTCC Kpn I	Mutant	This study
tolC-P2	CTATCGTCATAGGTTGCGTTTTTCGGCTTC	Mutant	This study
tolC-P3	GAAGCCGAAAAACGCAACCTATGACGATAGCAATATGGGCCAG	Mutant	This study
tolC-P4	TCCGAGCTCTCAGTTACGGAAAGGGT Sac I	Mutant	This study
tolC-clo-U	GGCGTCGACATGCAAATGAAGAAATTG Sal Ι	Complement	This study
tolC-clo-D	CGGGAATTCTCAGTTACGGAAAGGGTT EcoR Ι	Complement	This study
tolC-che-U	AACACGCTGCTGAAAGAA	Checking PCR	This study
tolC-che-D	ACGGTTTGTACGACGCTA	Checking PCR	This study
rrsG F	TATTGCACAATGGGCGCAAG	qRT-PCR	[23]
rrsG R	ACTTAACAAACCGCCTGCGT	qRT-PCR	[23]
csgB F	CATAATTGGTCAAGCTGGGACTAA	qRT-PCR	[24]
csgB R	GCAACAACCGCCAAAAGTTT	qRT-PCR	[24]
csgD F	CCCGTACCGCGACATTG	qRT-PCR	[24]
csgD R	ACGTTCTTGATCCTCCATGGA	qRT-PCR	[24]

^aSubscripts F and R indicate forward primers and reverse primers.