Research Article
Xanthium strumarium L. Extracts Produce DNA Damage Mediated by Cytotoxicity in In Vitro Assays but Does Not Induce Micronucleus in Mice
Table 2
Mutagenicity testing of Xanthium strumarium extract in the Salmonella/microsome assay (TA 100 and TA 98).
| Concentration (g/placa) |
TA 100 Mean revertants/plate ± SD |
TA 98 Mean revertants/plate ± SD | −S9 | +S9 | −S9 | +S9 |
| 0a | 58.33 ± 16.56 | 62.60 ± 15.1 | 25.67 ± 3.79 | 38.33 ± 2.52 | 50 | 54.33 ± 5.13 | 64.10 ± 10.5 | 26.00 ± 4.36 | 38.67 ± 11.85 | 150 | 45.33 ± 4.73 | 60.04 ± 13.7 | 28.67 ± 7.64 | 45.67 ± 8.33 | 500 | 44.00 ± 2.00 | 60.33 ± 5.77 | 22.00 ± 5.29 | 35.67 ± 20.11 | 1500 | 46.67 ± 2.52 | 70.70 ± 7.37 | 26.00 ± 4.36 | 40.67 ± 8.62 | 5000 | 51.67 ± 3.21 | 67.31 ± 2.52 | 27.00 ± 2.65 | 38.33 ± 2.52 | NaA1.5b | 480.0 ± 22.6** | | | | 2AF 20b | | 2280.0 ± 169.7** | | 3300.0 ± 141.4** | PA 100b | | | 992.0 ± 11.3** | |
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+S9: with hepatic fraction S9; −S9: without hepatic fraction S9. Negative control, dimethyl sulfoxide (DMSO). Positive controls. NaA: sodium azide; 2AF: 2-aminofluorene; PA: pichrolonic acid. P < 0.01 (Student’s t-test).
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