Molecular Analysis of VanA Outbreak of Enterococcus faecium in Two Warsaw Hospitals: The Importance of Mobile Genetic Elements

Table 1

Primers used in the analysis of Tn1546 transposon.

Primer pair	Primer names	Sequence (5′-3′)	Position in Tn1546	Application in this study	Reference
1	vanRSHAX-1	AGACAAGTCTGAGATTGACCTTGCC	4141–4165	PCR	[40]
	vanRSHAX-2	ATATGCTTCAAACCCACTGTTTTCC	8565–8589	PCR	[40]
2	Tn1546	GGAAAATGCGGATTTACAACGCTAAG	13–38	PCR	[40]
	ORF1-5	CACGTCCTGCCGACTATGATTATTT	1900–1876	PCR	[41]
3	ORF2-F	TCATTCCATTTCTGTATTTTCAATTT	3050–3086	PCR	[42]
	ORF2-R	GCCCATTAGCGGAATACAGA	3770–3751	PCR	[42]
4	ORF2-F2	ACTAATGTATCTAGGGCTTCA	3710–3731	PCR	[42]
	vanR-R	GCAATTTCATGTTCATCATCCA	4000–3979	PCR	[42]
5	vanS	AACGCTATTCCAAACTAGAA	4690–4710	PCR, sequencing	[41]
	vanS-R	GTCGGAAGCTCTACCCTAAA	5760–5741	PCR, sequencing	[41]
6	vanS1	ATTGTTCAGCATGGAGGGC	5700–5719	PCR, sequencing	[34]
	vanH2	GAGCATGGAATGCATCTGCC	6060–6041	PCR	[34]
7	vanA1	CATGAATAGAATAAAAGTTGCAATA	6978–7002	PCR	[20]
	vanX2	TTATTTAACGGGGAAATC	8600–8583	PCR, sequencing	[43]
8	vanX-F	ATGGGTATTTTCAGAAGTCCC	8580–8601	PCR, sequencing	[42]
	vanZ2	AATGGGTACGGTAAACGAGC	10555–10536	PCR	[34]
ORF1-4		GCATGTAGTGATGAAACACCTAGCTGC	960–987	sequencing	[41]
vanA2		CCCCTTTAACGCTAATACCATCAA	8007–7894	sequencing	[20]
vanY1		AGAGACGAACCATACCCCAA	9200–9181	sequencing	[42]
vanY2-R		AGTATGTGTTGATCCGGGAAAC	9900–9922	sequencing	this study