Research Article
Molecular Analysis of VanA Outbreak of Enterococcus faecium in Two Warsaw Hospitals: The Importance of Mobile Genetic Elements
Table 1
Primers used in the analysis of Tn1546 transposon.
| Primer pair | Primer names | Sequence (5′-3′) | Position in Tn1546 | Application in this study | Reference |
| 1 | vanRSHAX-1 | AGACAAGTCTGAGATTGACCTTGCC | 4141–4165 | PCR | [40] | vanRSHAX-2 | ATATGCTTCAAACCCACTGTTTTCC | 8565–8589 | PCR | [40] | 2 | Tn1546 | GGAAAATGCGGATTTACAACGCTAAG | 13–38 | PCR | [40] | ORF1-5 | CACGTCCTGCCGACTATGATTATTT | 1900–1876 | PCR | [41] | 3 | ORF2-F | TCATTCCATTTCTGTATTTTCAATTT | 3050–3086 | PCR | [42] | ORF2-R | GCCCATTAGCGGAATACAGA | 3770–3751 | PCR | [42] | 4 | ORF2-F2 | ACTAATGTATCTAGGGCTTCA | 3710–3731 | PCR | [42] | vanR-R | GCAATTTCATGTTCATCATCCA | 4000–3979 | PCR | [42] | 5 | vanS | AACGCTATTCCAAACTAGAA | 4690–4710 | PCR, sequencing | [41] | vanS-R | GTCGGAAGCTCTACCCTAAA | 5760–5741 | PCR, sequencing | [41] | 6 | vanS1 | ATTGTTCAGCATGGAGGGC | 5700–5719 | PCR, sequencing | [34] | vanH2 | GAGCATGGAATGCATCTGCC | 6060–6041 | PCR | [34] | 7 | vanA1 | CATGAATAGAATAAAAGTTGCAATA | 6978–7002 | PCR | [20] | vanX2 | TTATTTAACGGGGAAATC | 8600–8583 | PCR, sequencing | [43] | 8 | vanX-F | ATGGGTATTTTCAGAAGTCCC | 8580–8601 | PCR, sequencing | [42] | vanZ2 | AATGGGTACGGTAAACGAGC | 10555–10536 | PCR | [34] | ORF1-4 | GCATGTAGTGATGAAACACCTAGCTGC | 960–987 | sequencing | [41] | vanA2 | CCCCTTTAACGCTAATACCATCAA | 8007–7894 | sequencing | [20] | vanY1 | AGAGACGAACCATACCCCAA | 9200–9181 | sequencing | [42] | vanY2-R | AGTATGTGTTGATCCGGGAAAC | 9900–9922 | sequencing | this study |
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