Molecular Analysis of VanA Outbreak of Enterococcus faecium in Two Warsaw Hospitals: The Importance of Mobile Genetic Elements

Table 3

Plasmid profiles and colocalization of particular genes on vanA and other plasmids among selected 27 VREfm isolates.

Letter code	Isolate	MT/PT/ST	vanA plasmids in kb (hybridizing probes)	Other plasmids in kb (hybridizing probes)
IO (𝑁=11)
A	988^#	1/2-A/17	100 (rep17, pilA)	<30, 150 (pilA), 180, 270
B	989^*#	159/3/78	<30, 200	110 (rep2, rep17, pilA, axe-txe), 175, 230 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, aac (6′)-Ie-aph (2′′)-Ia, pilA), 310, >400
C	990^#	1/4-A/18	100 (rep17, rep18, pilA)	175, 230 (rep18), 280, 340
D	991^#	3/1-A/18	100 (rep17, pilA)	65 (𝑟𝑒𝑝𝑝𝑀𝐺1), 240 (𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, pilA), 295
E	3612^#	3/1-C/nd	100 (rep17, pilA)	70 (𝑟𝑒𝑝𝑝𝑀𝐺1), 235 (𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, pilA), 290
F	3620^#	10/5/262	100 (rep17, pilA)	65, 150 (pilA), 290
G	3622^#	3/1-B/18	100 (rep17, pilA)	70 (𝑟𝑒𝑝𝑝𝑀𝐺1), 240 (𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, pilA), 290
H	3623^#	3/1-C/nd	100 (rep17, pilA)	70 (𝑟𝑒𝑝𝑝𝑀𝐺1), 235 (𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, pilA), 290
I	3624	10/5/262	100 (rep17, pilA)	40, 65 (rep2, ω-ε-ζ), 150 (pilA), 290
J	3628^#	3/1-C/nd	100 (rep17, pilA)	70 (𝑟𝑒𝑝𝑝𝑀𝐺1), 235 (𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, pilA), 290
K	3629^#	7/2-B/18	100 (rep17, pilA)	235 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, hyl, aac (6′)-Ie-aph (2′′)-Ia, pilA), 270
IH (𝑁=16)
L	3549^#	11/7-A/202	100 (rep17, pilA)	70 (𝑟𝑒𝑝𝑝𝑀𝐺1, pilA), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA), 310
M	3550^x#	7/8/18	45 (rep2, rep17, rep18, ω-ε-ζ)	60 (rep17, pilA), 70, 195 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA), 230 (rep18), 315
N	3551^#	159/9/78	45 (rep17)	40 (rep2, ω-ε-ζ), 70 (rep17, pilA, axe-txe), 100, 190 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia), 240 (rep18), 290
O	3552^*#	7/10/18	<30, 170	65 (rep17, pilA), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, pilA, ω-ε-ζ)
P	3554	159/6-A/192	50 (rep17)	40 (rep2, ω-ε-ζ), 75 (rep17, pilA, axe-txe), 100 (pilA), 220 (aac (6′)-Ie-aph (2′′)-Ia), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
Q	3555	159/6-B/nd	50 (rep17)	40 (ω -ε-ζ), 75 (rep17, pilA, axe-txe), 100 (pilA), 220 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia), 240 (rep18)
R	3556	159/6-C/nd	50 (rep17)	40 (ω -ε-ζ), 75 (rep17, pilA, axe-txe), 100 (pilA), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
S	3557^x#	296/11/17	100 (rep17, pilA)	40 (ω -ε-ζ), 235 (pilA)
T	3558	159/6-A/nd	50 (rep17)	40 (rep2, ω-ε-ζ), 75 (rep17, pilA, axe-txe), 100 (pilA), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
U	3559	159/6-D/nd	50 (rep17)	40 (ω -ε-ζ), 75 (rep17), 100 (rep17), 240 (rep18, aac (6′)-Ie-aph (2′′)-Ia, pilA), 330 (𝑟𝑒𝑝𝑝𝐿𝐺1, axe-txe, aac (6′)-Ie-aph (2′′)-Ia, pilA)
V	3560^#	1/7-B/18	100 (rep17, 𝑟𝑒𝑝𝑝𝐿𝐺1, pilA)	85 (pilA), 235 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
W	3561^#	11/4-C/202	100 (rep17, 𝑟𝑒𝑝𝑝𝐿𝐺1, pilA)	240 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
X	3562	1/4-B/18	100 (rep17, pilA)	240 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
Y	3563	159/6-E/192	50 (rep17), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, pilA, aac(6′)-Ie-aph(2′′)-Ia)	40 (rep2, ω-ε-ζ), 75 (rep17, pilA, axe-txe), 100 (pilA), 240 (rep18, 𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia, pilA)
Z	3564^y#	7/12/18	70 (rep2, pilA, ω-ε-ζ)	60 (𝑟𝑒𝑝𝑝𝐿𝐺1), 75 (rep17, axe-txe), 130 (rep18), 200 (𝑟𝑒𝑝𝑝𝐿𝐺1, aac (6′)-Ie-aph (2′′)-Ia), 250
a	3567^y#	144/13/18	315 (rep17, 𝑟𝑒𝑝𝑝𝐿𝐺1, pilA)	75 (axe-txe, aac (6′)-Ie-aph (2′′)-Ia), 240 (rep18)

Letter code of each isolate corresponds to the designation used in Figure 2; nd: not determined; two isolates with the prototype Tn1546 marked with an asterisk; ^x,yisolates from the same patients “X” and “Y”; ^#isolates positive in conjugation.