Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma Cells
Figure 1
NMDA-activated NO production in differentiated SH-SY5Y cells. SH-SY5Y cells were seeded in 6-well plates and differentiated for 6 days. The cells were pretreated with vehicle, 1 μM 1400W, or 2 μM vinyl-L-NIO for 24 h. The cells were washed with Mg2+-free Krebs-Henseleit buffer twice followed by exposure to untreated control or 1 mM NMDA with 1 mM arginine and 30 μM glycine in Mg2+-free Krebs-Henseleit buffer with the continuous presence of 1400W or vinyl-L-NIO for 1 h. The supernatant was collected for NO measurement using the DAN method. The data are the mean ± SEM ( in each group). versus vehicle; NS: no significant difference.
