Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma Cells
Figure 3
Effect of rADI on mitochondrial membrane potential in SH-SY5Y cells. The differentiated cells were washed twice with Mg2+-free Krebs-Henseleit buffer and treated with control, 1 mM NMDA in normal medium, rADI pretreated medium, 1 mM NMDA in rADI pretreated medium, or 1 mM NMDA in rADI pretreated medium replenished with 0.8 mM arginine for 24 h. The cells were incubated with 5 μM rhodamine 123 in the dark for 40 min. The mitochondrial membrane potential was measured by flow cytometry. (a) Representative flow charts. The black peaks represent the rhodamine fluorescence intensity of the indicated treatments, and the gray peaks represent the control. (b) The quantitative rhodamine fluorescence intensity presented as percentage of the control. The data are the mean ± SEM () versus control; arg: arginine.
