Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom
Figure 2
Enzymatic characterization of the protein. (a) Determination of minimum coagulant dose (MCD) of BpirSP-39 using a concentration-response curve. The time of formation of fibrin network was measured by chronometer and the samples were evaluated visually. (b) Evaluation of inhibitors’ action on the coagulation activity of BpirSP-39. (c) Evaluation of BpirSP-39 thermostability on the clotting of human citrated plasma. (d) Effect of protein on different chromogenic substrates. (e) Effect of metals on the enzymatic activity of serineprotease (column 1, Cu2+; column 2, Mn2+; column 3, Ba2+; and column 4, Ca2+). (f) Effect of serineprotease on platelet aggregation. Results are expressed as means ± SD of two individual experiments ().
