Angiotensin II Removes Kidney Resistance Conferred by Ischemic Preconditioning
Figure 2
Expression of AngII and AT1R in non-IPC and IPC kidneys. IPC or non-IPC mice were exposed to either 30 minutes of bilateral renal ischemia/reperfusion (I/R) or a sham-operation (Sham). 24 hours after second surgery, kidneys were harvested. (a) AngII and (c) AT1R expression were determined by western blot using anti-AngII antibody. The AngII band is predominant at ~40 kDa. GAPDH expression was used to confirm equal protein loading. The densities of the blots were quantified using the ImageJ program. Serially sectioned paraffin-embedded kidney tissues were immunostained with (b) AngII (brown) and (d) AT1R (brown) antibodies as described in Section 2. Arrows indicate AngII-positive proximal tubules. Hematoxylin was used to detect nuclei (blue). Pictures were taken of the outer medulla in kidneys. S indicates sham. Scale bar: 50 μm. Values are means ± SEM . versus sham in non-IPC; versus I/R in non-IPC.
