Rhubarb Tannins Extract Inhibits the Expression of Aquaporins 2 and 3 in Magnesium Sulphate-Induced Diarrhoea Model
Figure 6
Rhubarb tannins extract (RTE) reduces the protein expression of aquaporins (AQPs) 2 and 3 in magnesium sulphate- (MgSO4-) induced HT-29 cells by western blot. Cells were placed in 24-well plate or 100-mm dishes for 24 h in the presence of MgSO4 with or without RTE (control, MgSO4, and RTE- (20, 40, and 80 μg/mL) treatment groups, resp.). ((a) and (b)) Expression levels of AQPs 2 and 3 protein in HT-29 cells treated by RTE (80 μg/mL) for different time (0, 3, 6, 12, and 24 h, resp.). ((c) and (d)) Expression levels of AQPs 2 and 3 proteins in HT-29 cells treated by RTE at the dosages of 20, 40, and 80 μg/mL for 24 h, respectively. Data are represented as the mean ± SD. ### versus normal control group. , , and versus vehicle group, respectively. in each group and each assay was repeated 3 times.
