Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells
Figure 1
Isolation and characterization of exosomes from SW780 bladder cancer cells. (a) Representative Western blot of exosomal markers in exosomes (exo) isolated from SW780 whole cell extract (WCE) and exosomes. Western blots were repeated three times. (b) Morphological characterization of exosomes isolated from SW780 cells by transmission electron microscopy at 40,000x magnification. TEM was performed at least three times. A representative image is shown. Scale bar shows 100 nm. Arrows indicate exosomes. (c) Representative image of labeled exosomes from SW780 cell exosomes, which were not detectable using the brightfield signal, but were using the PKH26 signal. (d) Correlation between SW780 cell number and number of exosomes secreted by these cells. value was calculated using GraphPad Prism software. Data is represented as average ± SEM.
