Research Article

Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells

Figure 3

Exosome uptake in bladder cancer cells increases with longer incubation times. Labeled SW780 exosomes were incubated with SW780 cells for the indicated times. This experiment was performed at least three times, with data from a representative experiment shown. A minimum of 10,000 cells were analyzed for each sample. (a) Representative images. (b) Spot count of internalized labeled exosomes. (c) Calculation of percent uptake based on the number of exosomes added, the number of spots per cell, and the total number of cells. (d) Fluorescence intensity of the labeled exosomes. Data is represented as average ± SEM.
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