Characterization of Uptake and Internalization of Exosomes by Bladder Cancer Cells
Figure 4
Exosomes are internalized by bladder cancer cells. SW780 cells were cultured with labeled exosomes for 4 hours, followed by detachment using either trypsin or calcium sequestration and labeling with CAR. This experiment was performed at least three times, with data from a representative experiment shown. A minimum of 10,000 cells were analyzed for each sample. (a) Representative images. (b) Spot count of labeled exosomes. (c) Fluorescence intensity of labeled exosomes. (d) Internalization wizard, measuring the ratio of the intensity inside the cell to the intensity of the entire cell. (e) Deconvolution microscopy with an actin (green) and PKH26-labeled exosomes (red). Actin staining was used as a cell marker in order to create a mask to determine whether exosomes were inside the cell or on the cell surface. The actin mask (left) shows that the exosomes are internalized. Arrows indicate exosomes. Data is represented as average ± SEM.
