Molecular Epidemiology of Genogroup II Noroviruses Infection in Outpatients with Acute Gastroenteritis in Nanjing, China (2010–2013)

Table 1

Primer and probe oligonucleotides used for real-time quantitative RT-PCR for NoV GI and GII, sapovirus, rotavirus, human astrovirus, and enteric adenovirus.

Primer or probe	Sequence 5^′-3^′a	References
Cog 1F (GI)	cgY tgg atg cgl ttY cat ga
Cog 1R (GI)	ctt aga cgc cat cat cat tYa c
Ring 1A (GI)	FAM^b-aga tYg cga tcY cct gtc ca-BHQ^c
Ring 1B (GI)	FAM-aga tcg cgg tct cct gtc ca-BHQ	Trujillo et al. [12]
Cog 2F (GII)	caR gaR BcN atg tty agR tgg atg ag
Cog 2R (GII)	tcg acg cca tct tca ttc aca
Ring 2 (GII)	FAM-tgg gag ggc gat cgc aat ct-BHQ
SapoF	gct gtt scy act ggt gca
SapoP	FAM-cca atc saa tgt ccc tga ggc aat acg saa-BHQ	Gunson et al. [13]
SapoR	ggc atc ctg tcr ttc caa gca
NVP3-FDeg	acc atc twc acr tra ccc tc	Freeman et al. [14]
NVP3-R	ggt cac ata acg ccc c
NVP3-R1	ggt cac ata acg ccc cta ta
NVP3-Probe	FAM-atg agc aca ata gtt aaa agc taa cac tgt caa-BHQ
AV1	ccg agt agg atc gag ggt
AV2	gct tct gat taa atc aat ttt aa	Le Cann et al. [15]
AVs	FAM-ctt ttc tgt ctc tgt tta gat tat ttt aat cac c-TAMRA^d
Adeno.fwd	ttc cag cat aat aac tcw ggc ttt g	Logan et al. [16]
Adeno.rev	aat ttt ttc tgw gtc agg ctt gg
Adeno.probe1	FAM-cca tac ccc ctt att gg-TAMRA
Adeno.probe2	FAM-cct tac ccc ctt att gg-TAMRA

aR = A or G, Y = C or T, N = any, W = T, U or A.
^bFAM, 6-carboxyfluorescein reporter dye.
^cBHQ, black hole quencher dye.
^dTAMRA, quencher dye.