Differential Expression of Cholecystokinin A Receptor in Gallbladder Cancer in the Young and Elderly Suggests Two Subsets of the Same Disease?

Table 5

CCKAR expression profile in human malignancies: literature review. CCKAR: cholecystokinin type A receptor.

First author	Tumour types	Number of samples	Methodology	Findings
Okada [19]	Gastric	14	RT-PCR	Suggest a greater role for CCK and CCKAR than for gastrin and CCK-B receptor in gastric cancers
Clerc [15]	Oesophageal, gastric, and colon cancer	8: oesophageal; 12: colon; 8: gastric	RT-PCR	The expression of CCKAR may be an important indicator of the influence of CCK on the origin and growth of these cancers
Reubi [20]	Various human malignancies	32: gastroenteropancreatic tumour 24: medullary thyroid carcinoma; 16: neuroblastoma; 27: meningioma; 65: breast carcinoma	Receptor autoradiography	CCKAR rarely expressed in tumors except gastroenteropancreatic tumors; (38%), meningiomas (30%), and some neuroblastomas (19%)
Weinberg [21]	Pancreatic cancer	22	RT-PCR	Overexpression of CCKAR mRNA in pancreatic cancer
Moonka [16]	Pancreatic cancer	30	RT-PCR	Increased expression of CCKAR mRNA may stimulate pancreatic cancer
Schulz [22]	Various human tumours	5: colorectal; 5: pancreatic adenocarcinoma; 5: breast; 10: ovarian; 4: prostate; 6: thyroid; 15: carcinoid; 8: pancreatic insulinoma; 4: pituitary adenoma; 4: pheochromocytoma; 4: glioblastoma; 4: meningioma	IHC	CCKAR overexpression in a subset of human neuroendocrine tumours may provide a molecular basis for efficient targeting of these tumors with radiolabeled CCK analogs
Rai [23]	Gallbladder cancer	94	IHC	Significant increase in expression of CCKAR in gallbladder cancer as compared to gallstone disease
Current study	Gallbladder cancer	31 resected samples	RT-PCR, real-time PCR, and ELISA	Overexpression of CCKAR mRNA and protein in GBC tissues as compared with normal gallbladder suggests its therapeutic potential

RT-PCR: reverse transcriptase-polymerase chain reaction and IHC: immunohistochemistry.