Activation of the AT1R/HIF-1α/ACE Axis Mediates Angiotensin II-Induced VEGF Synthesis in Mesenchymal Stem Cells

Figure 2

Activation of HIF-1α after Ang II-stimulated VEGF synthesis. (a) Western blot analysis of HIF-1α expression. MSCs were exposed to Ang II (100 nM) for 2 h, 4 h, or 6 h. , . (b) Western blot analysis of HIF-1α siRNA efficiency. , . (c) Real-time qPCR of VEGF mRNA level, . (d) ELISA of VEGF secretion. MSCs were pretreated by HIF-1α siRNA before being exposed to Ang II (100 nM) for 12 h. . .

(a)

(b)

(c)

(d)