Activation of the AT1R/HIF-1α/ACE Axis Mediates Angiotensin II-Induced VEGF Synthesis in Mesenchymal Stem Cells

Figure 3

Interaction of HIF-1α with ACE in MSCs. (a) Real-time qPCR analysis of ACE mRNA level. MSCs were pretreated by HIF-1α siRNA before being exposed to Ang II (100 nM) for 12 h. , . (b) Western blot analysis of ACE expression. MSCs were pretreated by HIF-1α siRNA before being exposed to Ang II (100 nM) for 6 h. , . (c) Western blot analysis of HIF-1α. MSCs were pretreated with or without captopril (1 μM) for 1 h before they were exposed to Ang II (100 nM) for 12 h. , .

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