Effect of Acute Gamma Irradiation on Curcuma alismatifolia Varieties and Detection of DNA Polymorphism through SSR Marker
Table 11
SSR primers, number of alleles, product size, expected heterozygosity, observed heterozygosity, number of effective alleles, Nei's gene diversity, Shannon’s Index, and PIC.
Locus
Primer sequence (5′–3′)
Repeat motif
Dose (Gy)
Range size (bp)
(°C)
(°C)
Ne
PIC
Clon01
F: ACTGGACTGTCCGAGAGCAT R: TCGTTTAGCGACAACGGATT
(TA)6TTG(TC)16
0
4
194–212
60.7
68–58
0.78
0.00
4.00
0.75
1.38
0.71
10
5
194–212
60.7
68–58
0.78
0.00
4.25
0.76
1.51
0.72
20
6
194–216
60.7
68–58
0.81
0.04
4.80
0.78
1.64
0.73
Clon04
F: TAAATTTGCGAAGGCAATCC R: CCGCAGAGGAATTTGAAGAG
(TATAG)2(AG)17
0
2
179–200
58.3
65–55
0.22
0.25
1.28
0.21
0.37
0.19
10
3
179–200
58.3
65–55
0.28
0.24
1.38
0.27
0.52
0.25
20
6
170–200
58.3
65–55
0.58
0.21
2.31
0.56
1.21
0.54
Clon08
F: CCGGTGAGGGTGATATCTTG R: AAGCTCAAGCTCAAGCCAAT
(GT)10
0
4
245–274
60.7
68–58
0.67
0.75
2.91
0.65
1.21
0.61
10
7
231–274
60.7
68–58
0.79
0.56
4.57
0.78
1.67
0.75
20
7
245–274
60.7
68–58
0.79
0.39
4.42
0.78
1.65
0.75
Clon09
F: GGAGGAGGCAGTTGATTTGT R: GCTTTGGTGGCTAGAGATGC
(AC)14
0
2
182–188
60.4
67–57
0.38
0.00
1.60
0.37
0.56
0.31
10
3
182–200
60.4
67–57
0.40
0.04
1.65
0.39
0.64
0.33
20
3
182–195
60.4
67–57
0.51
0.00
1.99
0.50
0.84
0.43
Clon10
F: GTGGGAATTGGATTGCTCTC R: GAGAACTCCCCATGCTTCAG
(GT)7
0
4
204–232
60.7
68–58
0.67
0.25
2.91
0.65
1.21
0.61
10
5
200–232
60.7
68–58
0.66
0.20
2.86
0.65
1.27
0.61
20
6
200–232
60.7
68–58
0.71
0.26
3.38
0.71
1.40
0.65
Clon11
F: GGGCTTTGTTTAGTTGTCGTG R: CAGGAATGAAGTCGGCAAC
(AGA)8
0
2
160–167
60.7
68–58
0.51
0.00
2.00
0.50
0.69
0.37
10
4
163–182
60.7
68–58
0.64
0.00
2.72
0.63
1.13
0.56
20
5
163–182
60.7
68–58
0.62
0.00
2.58
0.61
1.12
0.54
Clon12
F: GATTGGATCACATGGTGTGC R: TGGGTTGATGGTTTCTCTGTT
(CT)20
0
5
195–231
59.0
66–76
0.71
0.50
3.20
0.68
1.38
0.65
10
6
195–231
59.0
66–76
0.73
0.28
3.52
0.71
1.45
0.67
20
7
195–255
59.0
66–76
0.78
0.56
4.39
0.77
1.70
0.75
Clon14
F: TCAGTCGAGGGGTTCCTACT R: GAGAGCTGATCGCAAAAACC
(CTT)7
0
2
175–181
60.7
68–58
0.38
0.00
1.60
0.37
0.57
0.31
10
3
171–181
60.7
68–58
0.55
0.00
2.21
0.54
0.91
0.47
20
3
171–181
60.7
68–58
0.51
0.00
2.1
0.51
0.81
0.42
Total
0
25
10
36
20
41
Mean
0
3.1
0.47
10
4.5
0.54
20
5.1
0.61
na: number of alleles : observed heterozygosity; : expected heterozygosity; Ne: effective number of alleles; : Nei’s gene diversity; : Shannon’s index; PIC: polymorphic information content.