Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

<table>Investigation of primer binding to the genomic DNA (gDNA) of various species and influence of gDNA existence on ssDNA_ODN detection. Isolated gDNA was spiked with 2.5 and 5 pg of ssDNA_ODN, and the determined Ct values were compared with the Ct values without the addition of gDNA (called standard).</table>

BioMed Research International

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Figure 2

Figure 2: Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes 