Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

<table>Examination of ssDNA_ODN amounts in the concentrate and flow-through after centrifugation of water or 5 M urea solutions containing 10 <i>μ</i>g ssDNA_ODN using Amicon Ultra 30K centrifugal filters. Samples of 15 <i>μ</i>L were analyzed on a 10% denaturing urea-polyacrylamide gel. Lane 1: 300 ng ssDNA_ODN (positive control), Lane 2: concentrate of water solution, Lane 3: flow-through of water solution, Lane 4: concentrate of 5 M urea solution, and Lane 5: flow-through of 5 M urea solution.</table>

BioMed Research International

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Figure 3

Figure 3: Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes 