Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes
Figure 4
Comparison of ssDNA_ODN elution efficiency of water or 5 M urea solution at 93°C from the surface of test objects. Using qPCR, detectable amounts of ssDNA_ODN were determined after the elution of immobilized ssDNA_ODN in water or 5 M urea solution (). The ssDNA_ODN amount (5 μg) used for coating of test objects was added to each elution solution. The detected ssDNA_ODN amounts after the Amicon Ultra 30K centrifugal filter purification and concentration were set to 100% (positive controls). The results are presented relative to the positive control as means ± SEM.
