Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Table 1

Determined residual content of ssDNA_ODN (‰) from immobilized amount (5 𝜇g) on test objects after cleaning at different temperatures (60, 85, and 93°C) and times (10, 20, 25, and 30 min) with and without detergent (𝑛=3).

Cleaning temperature	Cleaning	Cleaning time
		10 min	20 min	25 min	30 min
60°C	With detergent	17.67	17.01	n.d.	n.d.
	Without detergent	41.80	56.36	23.15	14.85
85°C	With detergent	45.34	n.d.	n.d.	n.d.
	Without detergent	11.41	34.28	2.49	9.38
93°C	With detergent	0.43	0.22	n.d.	n.d.
	Without detergent	5.31	0.36	0.19	0.14

n.d.: not detectable.