Detection of Newcastle Disease Virus Minor Genetic Variants by Modified Single-Stranded Conformational Polymorphism Analysis

Figure 3

MSSCP electrophoresis of 123 nt NDV fusion protein fragments RT-PCR and PCR products. Lanes 2–4 are RT-PCR products (genomic RNA—nR template; L—La Sota strain, R—Roakin strain, and I—Italy strain) where lanes 5–19 are PCR products (plasmids with cloned fusion protein gen fragment—nD and aD templates). The PCR strains sample composition, plasmid ratio, and color code explanation can be found in the table below the figure. MSSCP was performed in 11% polyacrylamide gel without any additives as described in Table 2.